Double lysis: an integrative time-saving method yielding high-quality RNA from strawberry

High Yielding Microbubble Production Method.

Microfluidic approaches to microbubble production are generally disadvantaged by low yield and high susceptibility to (micro)channel blockages. This paper presents an alternative method of producing microbubbles of 2.6 μm mean diameter at concentrations in excess of 30 × 10(6) mL(-1). In this method, the nitrogen gas flowing inside the liquid jet is disintegrated into spray of microbubble when ...

High Quality RNA Isolation from Leaf, Shell, Root Tissues and Callus of Hazelnut (Corylus avellana L.)

Extraction of high quality RNA is a critical step in molecular genetics studies. Hazelnut is one of the most important nuts plants in the world. The presence of the taxol and other taxanes in hazelnut plant necessitates explaining their biosynthesis pathway and identifying the candidate genes. Therefore, an easy and practical method is necessary for RNA extraction from hazelnuts. Hazelnut has h...

Isolation of high-quality RNA from a wide range of woody plants

Isolation of high-quality RNA is one of the most crucial methods in molecular biology. RNA extraction from woody plants has been problematic due to the presence of rigid and woody tissues, large amounts of polysaccharides, polyphenols and other secondary metabolites. Here we present a suitable protocol for RNA isolation from a wide range of woody plants that includes eight gymnosperms and four ...

The challenge of getting a high quality of RNA from oocyte for gene expression study

The extraction of intact RNA from oocyte is quite challenging and time-consuming. A standard protocol using commercial RNA extraction kit, yields a low quantity of RNA in oocytes. In the past, several attempts in getting RNA for gene expression study ended up with a few different modified methods. Extraction of high-quality RNA from oocyte is important before further downstream analyses such as...

Rapid method for high-quality RNA isolation from seed endosperm containing high levels of starch.